97 datasets found
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Federal
Genomic-scale measurement of mRNA turnover and the mechanisms of action of the anti-cancer drug flavopiridol
U.S. Department of Health & Human Services —
Background Flavopiridol, a flavonoid currently in cancer clinical trials, inhibits cyclin-dependent kinases (CDKs) by competitively blocking their ATP-binding pocket.... -
Federal
Model-based cluster analysis of microarray gene-expression data
U.S. Department of Health & Human Services —
Background Microarray technologies are emerging as a promising tool for genomic studies. The challenge now is how to analyze the resulting large amounts of data.... -
Federal
Analysis of a human brain transcriptome map
U.S. Department of Health & Human Services —
Background Genome wide transcriptome maps can provide tools to identify candidate genes that are over-expressed or silenced in certain disease tissue and increase our... -
Federal
Receptor protein tyrosine kinase EphB4 is up-regulated in colon cancer
U.S. Department of Health & Human Services —
Background We have used commercially available cDNA arrays to identify EphB4 as a gene that is up-regulated in colon cancer tissue when compared with matched normal... -
Federal
Little qualitative RNA misexpression in sterile male F
U.S. Department of Health & Human Services —
Background Although the genetics of hybrid sterility has been the subject of evolutionary studies for over sixty years, no one has shown the reason(s) why alleles... -
Federal
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
U.S. Department of Health & Human Services —
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The... -
Federal
Manipulation of gene expression by an ecdysone-inducible gene switch in tumor xenografts
U.S. Department of Health & Human Services —
Background Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional... -
Federal
Vector algebra in the analysis of genome-wide expression data
U.S. Department of Health & Human Services —
Background Data from thousands of transcription-profiling experiments in organisms ranging from yeast to humans are now publicly available. How best to analyze these... -
Federal
Permutation-validated principal components analysis of microarray data
U.S. Department of Health & Human Services —
Background In microarray data analysis, the comparison of gene-expression profiles with respect to different conditions and the selection of biologically interesting... -
Federal
Survey of transcripts in the adult
U.S. Department of Health & Human Services —
Background: Classic methods of identifying genes involved in neural function include the laborious process of behavioral screening of mutagenized flies and then... -
Federal
Analysis of strain and regional variation in gene expression in mouse brain
U.S. Department of Health & Human Services —
Background We performed a statistical analysis of a previously published set of gene expression microarray data from six different brain regions in two mouse strains.... -
Federal
Evaluation of differential gene expression during behavioral development in the honeybee using microarrays and northern blots
U.S. Department of Health & Human Services —
Background The honeybee (Apis mellifera) has been used with great success in a variety of behavioral studies. The lack of genomic tools in this species has, however,... -
Federal
Normalization and analysis of DNA microarray data by self-consistency and local regression
U.S. Department of Health & Human Services —
A robust semi-parametric normalization technique has been developed, based on the assumption that the large majority of genes will not have their relative expression... -
Federal
Empirical characterization of the expression ratio noise structure in high-density oligonucleotide arrays
U.S. Department of Health & Human Services —
Background High-density oligonucleotide arrays (HDONAs) are a powerful tool for assessing differential mRNA expression levels. To establish the statistical... -
Federal
Expression profiles during honeybee caste determination
U.S. Department of Health & Human Services —
Background Depending on their larval environment, female honeybees develop into either queens or workers. As in other polyphenisms, this developmental switch depends... -
Federal
Genomic approaches to research in pulmonary hypertension
U.S. Department of Health & Human Services —
Genomics, or the study of genes and their function, is a burgeoning field with many new technologies. In the present review, we explore the application of genomic... -
Federal
Delayed union of femoral fractures in older rats:decreased gene expression
U.S. Department of Health & Human Services —
Background Fracture healing slows with age. While 6-week-old rats regain normal bone biomechanics at 4 weeks after fracture, one-year-old rats require more than 26... -
Federal
Obtaining reliable information from minute amounts of RNA using cDNA microarrays
U.S. Department of Health & Human Services —
Background High density cDNA microarray technology provides a powerful tool to survey the activity of thousands of genes in normal and diseased cells, which helps us... -
Federal
Gene expression profiles derived from fine needle aspiration correlate with response to systemic chemotherapy in breast cancer
U.S. Department of Health & Human Services —
Background Drug resistance in breast cancer is a major obstacle to successful chemotherapy. In this study we used cDNA microarray technology to examine gene... -
Federal
Identification of
U.S. Department of Health & Human Services —
Using cDNA microarray technology 12 new female-associated and 4 new male-associated gene transcripts were identified in mature adult Schistosoma mansoni parasites.
97 datasets found