Random amplification of polymorphic DNA: Difference between revisions

Revision as of 02:13, 17 May 2020

Revision as of 13:10, 9 November 2020

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{{forredirect|RAPD|the eye condition sometimes called Relative afferent pupillary defect (RAPD)|Marcus Gunn pupil}}

{{Refimprove|date=June 2016}}

'''RAPD''' (pronounced as "rapid") stands for ''''R'''andom '''A'''mplification of '''P'''olymorph'''i'''c '''D'''NA'.<ref>{{Cite web|url=http://www.rvc.ac.uk/review/dna_1/5_RAPD.cfm|title=rDNA: Random Amplification of Polymorphic DNA (RAPD)|website=www.rvc.ac.uk|access-date=2016-06-03}}</ref> It is a type of [[PCR]], but the segments of DNA that are amplified are random. The scientist performing RAPD creates several arbitrary, short primers (8–12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. By resolving the resulting patterns, a semi-unique profile can be gleaned from an RAPD reaction.