Optimized Multicolor Immunofluorescence Panel

Optimized Multicolor Immunofluorescence Panel, abbreviation OMIP, was a series of standardized multi-color flow cytometry panel launched by International Society for Advancement of Cytometry (ISAC), published in the form of columns in Cytometry Part A.^[1][2][3] The first two OMIP articles were published in September 2010 issue of this journal.^[4]

Purpose

The goals of OMIPs are: (1) to alleviate the development time for researchers who wish to use the same (or highly similar) panels, (2) to provide a starting point for the creation of novel OMIPs, and (3) to provide a mechanism for attribution to the developers of the panel via citation of the publication.^[1]

List

No.	color	Purpose	Species	Cell types	Ref
OMIP-001	16	T-cell cytokine production after in vitro stimulation and phenotyping of cytokine-producing T-cells	Human	PBMC	[5][4]
OMIP-002	16	Specificity and phenotype of CD8+ T-cells	Human	PBMC, bone marrow mononuclear cells	[6][4]
OMIP-003	12	Phenotyping memory B cells	human	PBMC, bone marrow, and tonsil mononuclear cell	[7]
OMIP-004	15	Determine Treg levels and to provide an in depth characterization of Tregs	Human	PBMC, ACK-lysed blood	[8]
OMIP-005	11	T cell cytokine production and memory phenotype	Rhesus macaque	PBMC	[9]
OMIP-006	9	Enumerating and phenotyping of T regulatory cells and subsets	Human	PBMC	[10]
OMIP-007	14	Characterize the phenotype, maturation, and activation of NK cells	Human	PBMC	[11]
OMIP-008	11	T cell cytokine production following in vitro stimulation	Human	PBMC, T cell clones/lines, tumor-resident T cell lines	[12]
OMIP-009	10	phenotype and function of CD4+ and CD8+ T-cells	Human	PBMC	[13]
OMIP-010	10	immunophenotyping of small hematopoietic cell	Human	Cerebrospinal fluid, bone marrow, peripheral blood	[14]
OMIP-011	8	Characterization of CECs in peripheral blood	Human	Whole blood, RBC-lysed and washed	[15]
OMIP-012	9	Human leukocyte reconstitution and phenotype	Humanized mouse	Whole blood, Ficoll-separated PBMC, splenocytes	[16]
OMIP-013	13	T-cell differentiation	Human	PBMC	[17]
OMIP-014	12	T-cell cytokine production and function after in vitro stimulation	Human	Cryopreserved PBMC	[18]
OMIP-015	13	T-reg characterization and T-cell activation	Human	PBMC	[19]
OMIP-016	10	Quality and phenotype of antigen-specific CD4 and CD8 T cells	Cynomolgus macaque or Human	Fresh or cryopreserved PBMCs	[20]
OMIP-017
OMIP-018
OMIP-019
OMIP-020
OMIP-021
OMIP-022
OMIP-023
OMIP-024
OMIP-025
OMIP-026					[21]
OMIP-027
OMIP-028
OMIP-029
OMIP-030
OMIP-031
OMIP-032					[22]
OMIP-033
OMIP-034
OMIP-035
OMIP-036
OMIP-037
OMIP-038
OMIP-039
OMIP-040
OMIP-041
OMIP-042
OMIP-043
OMIP-044
OMIP-045
OMIP-046
OMIP-047
OMIP-048
OMIP-049		Analysis of Human Myelopoiesis and Myeloid Neoplasms			[23]
OMIP-050	28				[24]
OMIP-051
OMIP-052
OMIP-053
OMIP-054
OMIP-055
OMIP-056
OMIP-057
OMIP-058
OMIP-059
OMIP-060
OMIP-061
OMIP-062	14	Myeloid and innate/adaptive lymphoid comprehensive immunophenotyping	Human	Whole peripheral blood, cord blood, PBMCs, nasal aspirate, and tracheal aspirate samples	[25]
OMIP-063
OMIP-064
OMIP-065
OMIP-066
OMIP-067
OMIP-068
OMIP-069
OMIP-070
OMIP-071
OMIP-072
OMIP-073
OMIP-074
OMIP-075
OMIP-076
OMIP-077
OMIP-078
OMIP-079
OMIP-080
OMIP-081
OMIP-082	25
OMIP-083	18
OMIP-084	28
OMIP-085	8
OMIP-086	22
OMIP-087
OMIP-088
OMIP-089	8
OMIP-090	18
OMIP-091	27
OMIP-092	6
OMIP-093	41
OMIP-094	24
OMIP-095
OMIP-096
OMIP-097
OMIP-098
OMIP-099
OMIP-100
OMIP-101	27

References

1. Mahnke, Yolanda; Chattopadhyay, Pratip; Roederer, Mario (September 2010). "Publication of optimized multicolor immunofluorescence panels". Cytometry Part A. 77A (9): 814–818. doi:10.1002/cyto.a.20916. PMID 20722004.
2. Tárnok, A (September 2016). "OMIPs start school". Cytometry. Part A. 89 (9): 795–6. doi:10.1002/cyto.a.22976. PMID 27657547.
3. Mahnke, Y; Tárnok, A (28 September 2020). "Celebrating 10 Years of OMIPs". Cytometry. Part A. doi:10.1002/cyto.a.24233. PMID 32985101.
4. Roederer, M; Tárnok, A (September 2010). "OMIPs--Orchestrating multiplexity in polychromatic science". Cytometry. Part A. 77 (9): 811–2. doi:10.1002/cyto.a.20959. PMID 20722007.
5. Mahnke, YD; Roederer, M (September 2010). "OMIP-001: Quality and phenotype of Ag-responsive human T-cells". Cytometry. Part A. 77 (9): 819–20. doi:10.1002/cyto.a.20944. PMID 20722005.
6. Chattopadhyay, PK; Roederer, M; Price, DA (September 2010). "OMIP-002: Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes". Cytometry. Part A. 77 (9): 821–2. doi:10.1002/cyto.a.20945. PMID 20722006.
7. Wei, C; Jung, J; Sanz, I (November 2011). "OMIP-003: phenotypic analysis of human memory B cells". Cytometry. Part A. 79 (11): 894–6. doi:10.1002/cyto.a.21112. PMC 3199331. PMID 21796774.
8. Biancotto, A; Dagur, PK; Fuchs, JC; Langweiler, M; McCoy JP, Jr (January 2012). "OMIP-004: in-depth characterization of human T regulatory cells". Cytometry. Part A. 81 (1): 15–6. doi:10.1002/cyto.a.21158. PMC 3510978. PMID 22052639.
9. Foulds, KE; Donaldson, M; Roederer, M (May 2012). "OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells". Cytometry. Part A. 81 (5): 360–1. doi:10.1002/cyto.a.22008. PMC 11389872. PMID 22438313.
10. Murdoch, DM; Staats, JS; Weinhold, KJ (April 2012). "OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry". Cytometry. Part A. 81 (4): 281–3. doi:10.1002/cyto.a.22024. PMC 3352600. PMID 22319016.
11. Eller, MA; Currier, JR (June 2012). "OMIP-007: phenotypic analysis of human natural killer cells". Cytometry. Part A. 81 (6): 447–9. doi:10.1002/cyto.a.22033. PMID 22411905.
12. Zuleger, CL; Albertini, MR (June 2012). "OMIP-008: measurement of Th1 and Th2 cytokine polyfunctionality of human T cells". Cytometry. Part A. 81 (6): 450–2. doi:10.1002/cyto.a.22035. PMC 3418807. PMID 22431369.
13. Lamoreaux, L; Koup, RA; Roederer, M (May 2012). "OMIP-009: Characterization of antigen-specific human T-cells". Cytometry. Part A. 81 (5): 362–3. doi:10.1002/cyto.a.22042. PMID 22438322.
14. Preijers, FW; Huys, E; Moshaver, B (June 2012). "OMIP-010: a new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples". Cytometry. Part A. 81 (6): 453–5. doi:10.1002/cyto.a.22056. PMID 22532446.
15. Lachmann, R; Lanuti, P; Miscia, S (July 2012). "OMIP-011: Characterization of circulating endothelial cells (CECs) in peripheral blood". Cytometry. Part A : the journal of the International Society for Analytical Cytology. 81 (7): 549–51. doi:10.1002/cyto.a.22071. PMID 22648996.
16. Long, BR; Stoddart, CA (August 2012). "OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice". Cytometry. Part A : the journal of the International Society for Analytical Cytology. 81 (8): 646–8. doi:10.1002/cyto.a.22074. PMID 22653780.
17. Mahnke, YD; Beddall, MH; Roederer, M (November 2012). "OMIP-013: differentiation of human T-cells". Cytometry. Part A : the journal of the International Society for Analytical Cytology. 81 (11): 935–6. doi:10.1002/cyto.a.22201. PMID 23027685.
18. De Rosa, SC; Carter, DK; McElrath, MJ (December 2012). "OMIP-014: validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining". Cytometry. Part A : the journal of the International Society for Analytical Cytology. 81 (12): 1019–21. doi:10.1002/cyto.a.22218. PMID 23081852.
19. Mahnke, YD; Beddall, MH; Roederer, M (February 2013). "OMIP-015: human regulatory and activated T-cells without intracellular staining". Cytometry. Part A : the journal of the International Society for Analytical Cytology. 83 (2): 179–81. doi:10.1002/cyto.a.22230. PMID 23166007.
20. Guenounou, S; Bosquet, N; Dembek, CJ; Le Grand, R; Cosma, A (February 2013). "OMIP-016: Characterization of antigen-responsive macaque and human T-cells". Cytometry. Part A : the journal of the International Society for Analytical Cytology. 83 (2): 182–4. doi:10.1002/cyto.a.22233. PMID 23184609.
21. Neumann, B; Sopper, S; Stahl-Hennig, C (September 2015). "OMIP-026: Phenotypic analysis of B and plasma cells in rhesus macaques". Cytometry. Part A. 87 (9): 800–2. doi:10.1002/cyto.a.22712. PMID 26115002.
22. Unsworth, A; Anderson, R; Haynes, N; Britt, K (June 2016). "OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland". Cytometry. Part A. 89 (6): 527–30. doi:10.1002/cyto.a.22867. PMID 27214375.
23. Zhu, G; Brayer, J; Padron, E; Mulé, JJ; Mailloux, AW (October 2018). "OMIP-049: Analysis of Human Myelopoiesis and Myeloid Neoplasms". Cytometry. Part A. 93 (10): 982–986. doi:10.1002/cyto.a.23598. PMC 6484443. PMID 30347519.
24. Nettey, L; Giles, AJ; Chattopadhyay, PK (November 2018). "OMIP-050: A 28-color/30-parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint Molecules". Cytometry. Part A. 93 (11): 1094–1096. doi:10.1002/cyto.a.23608. PMID 30347136.
25. Swieboda, D; Guo, Y; Sagawe, S; Thwaites, RS; Nadel, S; Openshaw, PJM; Culley, FJ (December 2019). "OMIP-062: A 14-Color, 16-Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples". Cytometry. Part A. 95 (12): 1231–1235. doi:10.1002/cyto.a.23907. PMC 6972618. PMID 31633878.