| Pos | Team | Pld | W | SOW | SOL | L | GF | GA | GD | Pts | Awards |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | St. Louis City 2 | 23 | 16 | 3 | 0 | 4 | 53 | 30 | +23 | 54 | Regular season champion |
| 2 | New York Red Bulls II | 23 | 14 | 2 | 2 | 5 | 54 | 46 | +8 | 48 | |
| 3 | Huntsville City FC | 22 | 11 | 4 | 2 | 5 | 46 | 25 | +21 | 43 | |
| 4 | Philadelphia Union II | 23 | 10 | 5 | 3 | 5 | 48 | 26 | +22 | 43 | |
| 5 | The Town FC | 20 | 12 | 2 | 2 | 4 | 49 | 26 | +23 | 42 | |
| 6 | Colorado Rapids 2 | 23 | 12 | 1 | 4 | 6 | 45 | 34 | +11 | 42 | |
| 7 | Chicago Fire FC II | 22 | 11 | 3 | 2 | 6 | 54 | 37 | +17 | 41 | |
| 8 | New England Revolution II | 21 | 10 | 5 | 1 | 5 | 40 | 27 | +13 | 41 | |
| 9 | Chattanooga FC | 23 | 10 | 4 | 3 | 6 | 35 | 33 | +2 | 41 | |
| 10 | Ventura County FC | 22 | 10 | 2 | 3 | 7 | 44 | 40 | +4 | 37 | |
| 11 | Real Monarchs | 21 | 8 | 4 | 3 | 6 | 38 | 35 | +3 | 35 | |
| 12 | Austin FC II (E) | 22 | 8 | 3 | 5 | 6 | 29 | 28 | +1 | 35 | |
| 13 | Toronto FC II (E) | 23 | 9 | 1 | 4 | 9 | 28 | 36 | −8 | 33 | |
| 14 | New York City FC II (E) | 23 | 8 | 2 | 4 | 9 | 44 | 46 | −2 | 32 | |
| 15 | Whitecaps FC 2 (E) | 22 | 8 | 3 | 1 | 10 | 47 | 43 | +4 | 31 | |
| 16 | Minnesota United FC 2 (E) | 22 | 8 | 2 | 3 | 9 | 36 | 37 | −1 | 31 | |
| 17 | North Texas SC (E) | 22 | 8 | 3 | 1 | 10 | 35 | 47 | −12 | 31 | |
| 18 | Orlando City B (E) | 23 | 8 | 2 | 2 | 11 | 32 | 46 | −14 | 30 | |
| 19 | Carolina Core FC (E) | 23 | 6 | 4 | 4 | 9 | 31 | 33 | −2 | 30 | |
| 20 | Los Angeles FC 2 (E) | 23 | 8 | 2 | 1 | 12 | 39 | 48 | −9 | 29 | |
| 21 | Houston Dynamo 2 (E) | 23 | 7 | 3 | 2 | 11 | 32 | 37 | −5 | 29 | |
| 22 | Crown Legacy FC (E) | 23 | 6 | 3 | 5 | 9 | 39 | 44 | −5 | 29 | |
| 23 | FC Cincinnati 2 (E) | 22 | 5 | 7 | 0 | 10 | 25 | 31 | −6 | 29 | |
| 24 | Portland Timbers 2 (E) | 22 | 7 | 2 | 3 | 10 | 34 | 40 | −6 | 28 | |
| 25 | Tacoma Defiance (E) | 21 | 7 | 2 | 2 | 10 | 51 | 53 | −2 | 27 | |
| 26 | Atlanta United 2 (E) | 21 | 5 | 2 | 6 | 8 | 29 | 34 | −5 | 25 | |
| 27 | Inter Miami CF II (E) | 22 | 6 | 0 | 4 | 12 | 34 | 53 | −19 | 22 | |
| 28 | Columbus Crew 2 (E) | 22 | 4 | 1 | 3 | 14 | 25 | 47 | −22 | 17 | |
| 29 | Sporting Kansas City II (E) | 22 | 2 | 1 | 3 | 16 | 21 | 55 | −34 | 11 |
Updated to match(es) played on August 24, 2025. Source: MLSNP
Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) fewer disciplinary points; 6) away goal differential; 7) away goals scored; 8) home goals differential; 9) home goals scored; 10) coin toss (2 clubs tied) or drawing of lots (≥3 clubs tied)
(E) Eliminated
Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) fewer disciplinary points; 6) away goal differential; 7) away goals scored; 8) home goals differential; 9) home goals scored; 10) coin toss (2 clubs tied) or drawing of lots (≥3 clubs tied)
(E) Eliminated