| Pos | Team | Pld | W | L | T | GF | GA | GD | Pts | Qualification |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Chattanooga Red Wolves SC | 20 | 11 | 2 | 7 | 33 | 19 | +14 | 40 | Playoffs |
| 2 | Spokane Velocity FC | 19 | 11 | 3 | 5 | 27 | 16 | +11 | 38 | |
| 3 | One Knoxville SC | 20 | 9 | 3 | 8 | 27 | 17 | +10 | 35 | |
| 4 | FC Naples | 21 | 9 | 5 | 7 | 28 | 23 | +5 | 34 | |
| 5 | Charlotte Independence | 21 | 8 | 6 | 7 | 36 | 34 | +2 | 31 | |
| 6 | AV Alta FC | 21 | 7 | 6 | 8 | 31 | 29 | +2 | 29 | |
| 7 | Texoma FC | 22 | 6 | 9 | 7 | 28 | 38 | −10 | 25 | |
| 8 | Union Omaha | 20 | 7 | 10 | 3 | 28 | 31 | −3 | 24 | |
| 9 | Greenville Triumph SC | 21 | 6 | 9 | 6 | 29 | 31 | −2 | 24 | |
| 10 | Portland Hearts of Pine | 18 | 5 | 4 | 9 | 21 | 19 | +2 | 24 | |
| 11 | Forward Madison FC | 21 | 4 | 7 | 10 | 20 | 22 | −2 | 22 | |
| 12 | Richmond Kickers | 21 | 5 | 11 | 5 | 28 | 35 | −7 | 20 | |
| 13 | South Georgia Tormenta FC | 21 | 5 | 11 | 5 | 29 | 40 | −11 | 20 | |
| 14 | Westchester SC | 20 | 3 | 10 | 7 | 31 | 42 | −11 | 16 |
Updated to match(es) played on August 27, 2025. Source: USL League One
Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) points earned against top four finishers; 6) disciplinary points; 7) drawing of lots.
Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) points earned against top four finishers; 6) disciplinary points; 7) drawing of lots.