User:AMYCREYNOLDS/Single cell analysis/AMYCREYNOLDS Peer Review

Peer review

This is where you will complete your peer review exercise. Please use the following template to fill out your review.

Peer Review Instructions:  Produce a summary of your findings. Copy the questions below to your Peer review page. IGNORE THE TEMPLATE that is pre-loaded on the Peer Review feedback page Add your answers in bold (300 words minimum).

1. Is it obvious to you which sections of the article have been revised? Is the new content relevant to the topic?
   • No, it is not obvious which sections have been revised. The sections are not labeled as what is from the original article and what is new. However, I saw on the peer-review assignment sheet that the section about Associated Technologies was a new section. To make it clear for future peer reviewers, be sure to distinguish between old and new material within the sandbox so the reviewer does not have to switch back and forth between the article and the sandbox .
   • However, the new content added "Associated Technologies" is relevant to the topic because it further explains the techniques used in single-cell isolation. I like that the information is explained all on one page, rather than having to click the link for each technology to determine what it does. I think this makes it much easier for someone to read. If someone does not have a biology background, it could make it more confusing to have to switch back and forth between Wiki pages to determine what words mean.
   • After further reading the article, I could tell that the technology was explained under the same section as “Single-Cell Isolation”. Therefore, the edits to the page broke up the section to clearly explain the technologies used. It makes it much easier to read. The information is definitely relevant to the topic.

2. What does the article do well? Is there anything from your review that impressed you? Any particular information that you found especially informative.

• I like how the original article broke down each new technology used in Single-Cell Analysis. I thought it made it very easy to follow how it listed the technology and subsections under each technology included techniques used and their purposes.
• I think that the section, Single-Cell Isolation, could greatly benefit from sections like the ones previously mentioned. I think that the newly added section in Asgnia’s sandbox→ Associated Technologies→ could be improved even more by further explaining the technologies and then explaining some techniques associated with each technology. Clearly defined sections will help the reader by breaking down the article into smaller sections.
• I found the explanation of each technology used in Single-Cell Isolation to be very informative. I am very unfamiliar with this topic. I can use all of the help I can get understanding the techniques and terminology used throughout the article.

3. What overall adjustments do you suggest the author apply to the article? Why would those changes be an improvement? What's the most important thing the author could do to improve the article?

• I noticed that in the new section, “Associated Technologies”, that enzymatic digestion, FACS, micromanipulation, and serial dilution were not included. However, they were listed as technologies used in Single-Cell Isolation. To be more thorough, I think that an explanation of all technologies should be added. I think that if a new section is made describing associated technology, then all of the technology should be described.

4. Did you notice anything about the article you reviewed that could be applicable to your own article? Let them know.

• No, I did not notice anything about the article that could be applicable in my own article. However, I found everything that I read to be very interesting.

5. Is all new content backed up by a reliable source of information?

• Yes, it is. All new content can be related to the article, “Technologies for Single-Cell Isolation”.

6. Are the sources fairly current (> 2015)? Check a few links. Do they work?

• The only new link added in the assigned Peer Review portion was to an article called “Technologies for Single-Cell Isolation”. The article is fairly current. It was published August 16, 2015. The link works.
• I found 14 of the 56 articles included in the original article to be older than 2015 (references 1,3,9,13,16,19,23,24,28,44,47,48,49 and 50). Even though these articles are older than 5 years, I feel like they are still valuable references for the article. All of the links work, despite the references being before 2015.

7. Summarize any typographical/grammatical errors that you found.

• a "trap" at a single given time, by passive microfluidic transport. → remove the comma before by

• Laser Capture Microdissection technique used in single-cell analysis, to dissect and separate individual cells or sections from tissue samples of interest, with a focused laser. → reword to say The Laser Capture Microdissection technique utilizes a laser to dissect and separate individual cells, or sections, from tissue samples of interest.

• The methods involve the observation of a cell under a microscope, so that a section for analysis can be identified and labeled so that the laser can cut the cell, and the cell can be extracted for analysis. → run on sentence. Break into more sentences. Could say: First, a cell must be observed under a microscope to identify and label a section for analysis. Then, the laser can cut the cell to be extracted for study.

• Manual single cell picking is a method where cells in a suspension are viewed under a microscope, and individually picked using a micropipette. → remove comma. No need for a comma before “and” if it is not a compound sentence.

• Original: Microfluidics this method allows for the isolation of individual cells for further proceeding analyses. The principles that outline the various microfluidic processes for single-cell separation: droplet-in-oil based isolation, pneumatic membrane valving, and hydrodynamic cell traps. Droplet-in-oil based microfluidics uses oil-filled channels to hold separated aqueous droplets, this allows the single cell to be contained and isolated from the inside the oil based channels. Pneumatic membrane valves uses the manipulating of air pressure, to isolate individual cells by membrane deflection. The manipulation of the pressure source allows the for opening or closing of channels in a microfluidic network. The system requires an operator, typically, and are limited in throughput.
  • Revised entire section/multiple sentences: Microfluidics allows for the isolation of individual cells for further analyses. The following principles outline the various microfluidic processes for single-cell separation: droplet-in-oil based isolation, pneumatic membrane valving, and hydrodynamic cell traps. Droplet-in-oil based microfluidics uses oil-filled channels to hold separated aqueous droplets. This allows the single cell to be contained and isolated from inside of the oil based channels. Pneumatic membrane valves use the manipulation of air pressure to isolate individual cells by membrane deflection. The manipulation of the pressure source allows the opening or closing of channels in a microfluidic network. Typically, the system requires an operator and is limited in throughput.

• Original: Raman tweezers is a technique where  Raman spectroscopy is combined with optical tweezers, which uses a laser beam to trap, and manipulate cells.
  • Revised: The technique of Raman tweezers combines the use of Raman spectroscopy and optical tweezers, which use a laser beam to trap and manipulate cells.

• Original: The development of hydrodynamic-based microfluidic biochips has been increasing over the years. In this technique, the cells or particles are trapped in a particular region for single cell analysis (SCA) usually without any application of external force fields such as optical, electrical, magnetic or acoustic. There is a need to explore the insights of SCA in the cell's natural state and development of these techniques is highly essential for that study. Researchers have highlighted the vast potential field that needs to be explored to develop biochip devices to suit market/researcher demands. Hydrodynamic microfluidics facilitates the development of passive lab-on-chip applications. A latest review gives an account of the recent advances in this field, along with their mechanisms, methods and applications.
  • Revised: The development of hydrodynamic-based microfluidic biochips has been increasing over the years. In this technique, the cells are trapped in a particular region for single cell analysis (SCA). This usually occurs without any application of external force fields such as optical, electrical, magnetic or acoustic. There is a need to explore the insights of SCA in the cell's natural state, and the development of these techniques is highly essential for that study. Researchers have highlighted the need to develop biochip devices to suit market and researcher demands. Hydrodynamic microfluidics facilitate the development of passive lab-on-chip applications. A recent review highlighted the current advances in this field, along with the mechanisms, methods and applications. → need to cite review… do not know which one you are referring to

8. Student authors are responsible for all images on their page (even if not part of their revised subsection). Double check the original page to make sure images are acceptable and clearly described. See associated tutorial to review Wiki image requirements. Summarize your findings.

• Only one image was included in the original article. It depicted a single cell performing the central dogma of biology. This is relevant to the article because single-cell analysis studies the genomics, transcriptomics, proteomics, and metabolics of the cell at a single cell level.

9. Identify at least one additional reference that you think may contribute to the article. Explain why you think this article would benefit from the new information. Be sure to provide the reference in your write-up.

• I think that it would be very helpful to the reader if more information defining single-cell isolation was included. Then, you can make the “Associated Technology” a sub-section.
• I think the article, Single-Cell Isolation and Analysis^[1], would provide important information to further explain single-cell isolation. I know that the article is already included in the 56 original references. However, I think that the information from this article should be used to create a more in-depth intro to single-cell isolation.

1. Hu, Ping; Zhang, Wenhua; Xin, Hongbo; Deng, Glenn (2016). "Single Cell Isolation and Analysis". Frontiers in Cell and Developmental Biology. 4. doi:10.3389/fcell.2016.00116. ISSN 2296-634X. PMC 5078503. PMID 27826548.