Lbates2008

I am adding onto the existing article on PLCg1. I think I'm going to add my paragraphs under the function tab:

Common to all PLC isozymes, PLCg1 consists of an N-terminal PH ___domain, which translocates PLC to the plasma membrane and binds PIP_{3 (}^[1]₎, four EF hands, an X and Y catalytic region comprising the TIM barrel, and a C-terminal C2 ___domain. Specific to the PLCg isozymes is a large separation between the X and Y domains consisting of a split PH ___domain, tandem SH2 domains, and an SH3 ___domain. The SH2 domains bind phosphorylated tyrosine residues on target proteins via their FLVR sequence motifs, activating the catalytic function of PLCg; and the SH3 ___domain binds to proline-rich sequences on the target protein (^[2]).

PLCg1 can be activated by receptor tyrosine kinases (RTKs) and non-receptor tyrosine kinases. For example, when activated, fibroblast growth factor receptor 1 and epidermal growth factor receptor are RTKs that, upon ligand binding, have phosphorylated tyrosine residues, which provide docking sites for PLCg1 SH2 domains (^[3]). The activated RTKs phosphorylate PLCg1 at tyrosines located at position 472, 771, 775, 783, and 1254^[4]. Non-receptor tyrosine kinases interact with PLCg1 in large complexes at the plasma membrane. For example, in T cells, Lck and Fyn (Src family kinases) phosphorylate immunoreceptor tyrosine-based activation motifs (ITAMs) on the T-cell antigen receptor (TCR). The phosphorylated ITAMs recruit ZAP-70, which phosphorylates tyrosines in LAT and SLP-76. PLCg1 binds to LAT through its n-terminal SH2 ___domain and to SLP-76 via its SH3 ___domain (^[3]).

This user is a student editor in Wikipedia:Wiki_Ed/Florida_Institute_of_Technology/Molecular_Genetics_(Spring_2017). Student assignments should always be carried out using a course page set up by the instructor. It is usually best to develop assignments in your sandbox. After evaluation, the additions may go on to become a Wikipedia article or be published in an existing article.

1. Singh, Shaneen M.; Murray, Diana. "Molecular modeling of the membrane targeting of phospholipase C pleckstrin homology domains". Protein Science. 12 (9): 1934–1953. doi:10.1110/ps.0358803. PMC 2323991. PMID 12930993.
2. Gresset, Aurelie; Hicks, Stephanie N.; Harden, T. Kendall; Sondek, John (2010-11-12). "Mechanism of Phosphorylation-induced Activation of Phospholipase C-γ Isozymes". Journal of Biological Chemistry. 285 (46): 35836–35847. doi:10.1074/jbc.M110.166512. ISSN 0021-9258. PMC 2975207. PMID 20807769.
3. Gresset, Aurelie; Sondek, John; Harden, T. Kendall (2012-01-01). Balla, Tamas; Wymann, Matthias; York, John D. (eds.). Phosphoinositides I: Enzymes of Synthesis and Degradation. Subcellular Biochemistry. Springer Netherlands. pp. 61–94. doi:10.1007/978-94-007-3012-0_3. ISBN 9789400730113. PMC 3638883. PMID 22403074.
4. Bae, Jae Hyun; Lew, Erin Denise; Yuzawa, Satoru; Tomé, Francisco; Lax, Irit; Schlessinger, Joseph. "The Selectivity of Receptor Tyrosine Kinase Signaling Is Controlled by a Secondary SH2 Domain Binding Site". Cell. 138 (3): 514–524. doi:10.1016/j.cell.2009.05.028. PMC 4764080. PMID 19665973.